{"id":1260,"date":"2025-02-19T02:00:31","date_gmt":"2025-02-19T02:00:31","guid":{"rendered":"https:\/\/autoclaveequipment.com\/?p=1260"},"modified":"2025-02-19T02:02:04","modified_gmt":"2025-02-19T02:02:04","slug":"autoclave-operation-guide","status":"publish","type":"post","link":"https:\/\/autoclaveequipment.com\/sl\/autoclave-operation-guide\/","title":{"rendered":"Protokol delovanja avtoklava: Postopek sterilizacije korak za korakom"},"content":{"rendered":"
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1. Priprava pred sterilizacijo<\/span><\/strong><\/span><\/h4>

1.1 Kontrolni seznam opreme<\/span><\/strong><\/span><\/p>

Artikel<\/span><\/span><\/th>Specifikacija<\/span><\/span><\/th>Metoda preverjanja<\/span><\/span><\/th><\/tr><\/thead>
\u010cisto\u010da prostora<\/span><\/span><\/td>\uff1c5 CFU\/cm\u00b2 ostanek<\/span><\/span><\/td>Test bioluminiscence ATP<\/span><\/span><\/td><\/tr>
Upornost vode<\/span><\/span><\/td>\u22651 M\u03a9-cm @25\u00b0C<\/span><\/span><\/td>Kalibracija merilnika prevodnosti<\/span><\/span><\/td><\/tr>
Celovitost tesnila vrat<\/span><\/span><\/td>Stisk 0,5-0,7 mm<\/span><\/span><\/td>Preskus razpadanja tlaka<\/span><\/span><\/td><\/tr><\/tbody><\/table><\/figure>

1.2 Priprava tovora<\/span><\/strong><\/span><\/p>

  • Zahteve za pakiranje: <\/span><\/p>

    • Tekstilni paketi: \u226430 cm \u00d7 30 cm \u00d7 50 cm <\/span><\/p><\/li>

    • Pladnji za instrumente: \u22647 kg suhe te\u017ee <\/span><\/p><\/li>

    • Posode za teko\u010dino: \u226475% razmerje polnjenja <\/span><\/p><\/li><\/ul><\/li>

    • Pravila orientacije: <\/span><\/p>

      \u00a0<\/div>
      \ud835\udc34\ud835\udc56\ud835\udc5f \ud835\udc45\ud835\udc52\ud835\udc5a\ud835\udc5c\ud835\udc63\ud835\udc4e\ud835\udc59 \ud835\udc38\ud835\udc53\ud835\udc53\ud835\udc56\ud835\udc50\ud835\udc56\ud835\udc52\ud835\udc5b\ud835\udc50\ud835\udc66=1-\ud835\udf0390(\ud835\udf03=\ud835\udc61\ud835\udc56\ud835\udc59\ud835\udc61 \ud835\udc4e\ud835\udc5b\ud835\udc54\ud835\udc59\ud835\udc52)<\/div>
      \u00a0<\/div><\/div>

      Za optimalno odstranjevanje zraka vzdr\u017eujte temperaturo \u03b8 \u226415\u00b0.<\/span><\/em><\/span><\/p><\/li><\/ul>

      <\/div>

      2. Protokol izvedbe cikla<\/span><\/strong><\/span><\/h4>

      2.1 Parametri faze<\/span><\/strong><\/span><\/p>

      Faza<\/span><\/span><\/th>Temperatura<\/span><\/span><\/th>Tlak<\/span><\/span><\/th>Trajanje<\/span><\/span><\/th>Kriti\u010dna kontrolna to\u010dka<\/span><\/span><\/th><\/tr><\/thead>
      Predvakuumiranje<\/span><\/span><\/td>50-60\u00b0C<\/span><\/span><\/td>-12 PSI<\/span><\/span><\/td>3\u00d7 impulzi<\/span><\/span><\/td>\uff1e98% odstranjevanje zraka<\/span><\/span><\/td><\/tr>
      Ogrevanje<\/span><\/span><\/td>121\u00b0C<\/span><\/span><\/td>15 PSI<\/span><\/span><\/td>\u22648 minut dviga<\/span><\/span><\/td>\uff1c1\u00b0C\/min prekora\u010ditev<\/span><\/span><\/td><\/tr>
      Sterilizacija<\/span><\/span><\/td>121-134\u00b0C<\/span><\/span><\/td>15-30 PSI<\/span><\/span><\/td>T = D\u00d76 + 5<\/span><\/span><\/td>Stabilnost \u00b10,5 \u00b0C<\/span><\/span><\/td><\/tr>
      Izpu\u0161ni plin<\/span><\/span><\/td>100\u00b0C<\/span><\/span><\/td>0 PSI<\/span><\/span><\/td>7-10 minut<\/span><\/span><\/td>\uff1c0.5% zadr\u017ei vlago<\/span><\/span><\/td><\/tr>
      Su\u0161enje<\/span><\/span><\/td>80-90\u00b0C<\/span><\/span><\/td>-5 PSI<\/span><\/span><\/td>25-40 minut<\/span><\/span><\/td>\uff1e95% suhost<\/span><\/span><\/td><\/tr><\/tbody><\/table><\/figure>

      2.2 Zaporedje vnosa parametrov<\/span><\/strong><\/span><\/p>

      1. Izberite vrsto cikla: <\/span><\/p>

        • Te\u017ea: 121\u00b0C\/30min <\/span><\/p><\/li>

        • Predvakuumiranje: 132 \u00b0C\/4 min <\/span><\/p><\/li>

        • Teko\u010dina: 115 \u00b0C\/30 min <\/span><\/p><\/li><\/ul><\/li>

        • Nastavite varnostne rezerve: <\/span><\/p>

          \u00a0<\/div>
          \ud835\udc47\ud835\udc60\ud835\udc52\ud835\udc61=\ud835\udc47\ud835\udc5f\ud835\udc52\ud835\udc5e+\ud835\udc36\u210e\ud835\udc4e\ud835\udc5a\ud835\udc4f\ud835\udc52\ud835\udc5f \ud835\udc49\ud835\udc5c\ud835\udc59\ud835\udc62\ud835\udc5a\ud835\udc52(\ud835\udc3f)\/1000<\/div>
          \u00a0<\/div><\/div>

          Primer: 100-litrska komora pri 121 \u00b0C \u2192 121 + 0,316 = 121,3 \u00b0C<\/span><\/em><\/span><\/p><\/li>

        • Za\u010detek cikla z dvojnim preverjanjem upravljavca<\/span><\/p><\/li><\/ol>

          <\/div>

          3. Potrjevanje po zaklju\u010dku cikla<\/span><\/strong><\/span><\/h4>

          3.1 Takoj\u0161nji pregledi<\/span><\/strong><\/span><\/p>

          • Pregled filtra za izpust komore (ostanek \uff1c1mL) <\/span><\/p><\/li>

          • Analiza izpisa: <\/span><\/p>

            Parameter<\/span><\/span><\/th>Sprejemljiv razpon<\/span><\/span><\/th><\/tr><\/thead>
            F\u2080 vrednost<\/span><\/span><\/td>\u226515 minut<\/span><\/span><\/td><\/tr>
            Padec temperature<\/span><\/span><\/td>\uff1c0,3 \u00b0C za \uff1c3 sekunde<\/span><\/span><\/td><\/tr>
            Zadr\u017eevanje tlaka<\/span><\/span><\/td>\u00b10,7 PSI od ciljne vrednosti<\/span><\/span><\/td><\/tr><\/tbody><\/table><\/figure><\/li><\/ul>

            3.2 Biolo\u0161ko spremljanje<\/span><\/strong><\/span><\/p>

            • Test Geobacillus stearothermophilus: <\/span><\/p>

              • Inkubacija: 56-60 \u00b0C za 24 ur <\/span><\/p><\/li>

              • Prebivalstvo: 1\u00d710\u2076 spor\/nosilec <\/span><\/p><\/li>

              • Merila za sprejem: Brez spremembe barve (indikator pH) <\/span><\/p><\/li><\/ul><\/li><\/ul>

                3.3 Merila za sprostitev obremenitve<\/span><\/strong><\/span><\/p>

                1. Sprejeti fizi\u010dni monitorji <\/span><\/p><\/li>

                2. Barvno usklajeni kemi\u010dni indikatorji <\/span><\/p><\/li>

                3. Biolo\u0161ki rezultati negativni <\/span><\/p><\/li>

                4. Potrjena suhost (\uff1c0,2% vlage) <\/span><\/p><\/li><\/ol>

                  <\/div>

                  Protokol vzdr\u017eevanja<\/span><\/strong><\/span><\/h3>

                  Dnevno:<\/span><\/strong><\/span><\/p>

                  • \u010ci\u0161\u010denje komore z detergentom z nevtralnim pH <\/span><\/p><\/li>

                  • Pregled tesnila vrat (zamenjajte, \u010de so razpoke \uff1e0,2 mm) <\/span><\/p><\/li><\/ul>

                    Tedensko:<\/span><\/strong><\/span><\/p>

                    • Preizkus funkcionalnosti parne pasti <\/span><\/p><\/li>

                    • Merjenje prevodnosti vode <\/span><\/p><\/li><\/ul>

                      Mese\u010dno:<\/span><\/strong><\/span><\/p>

                      • Kalibracija termo\u010dlena (toleranca \u00b10,25 \u00b0C) <\/span><\/p><\/li>

                      • Preskus stopnje vakuuma (\u226510 PSI\/min) <\/span><\/p><\/li><\/ul>

                        Letno:<\/span><\/strong><\/span><\/p>

                        • Tla\u010dni preskus v komori (1,5\u00d7MAWP za 10 min) <\/span><\/p><\/li>

                        • Certificiranje varnostnega ventila <\/span><\/p><\/li><\/ul>

                          <\/div>

                          Vodnik za odpravljanje te\u017eav<\/span><\/strong><\/span><\/h3>
                          Simptom<\/span><\/span><\/th>Diagnosti\u010dni postopek<\/span><\/span><\/th>Popravljalni ukrepi<\/span><\/span><\/th><\/tr><\/thead>
                          Mokri paketi<\/span><\/span><\/td>Preverite parametre faze su\u0161enja<\/span><\/span><\/td>Podalj\u0161anje \u010dasa su\u0161enja za 20%<\/span><\/span><\/td><\/tr>
                          nihanje temperature<\/span><\/span><\/td>Preverite kakovost pare (suhost \uff1e97%)<\/span><\/span><\/td>O\u010distite tuljave generatorja pare<\/span><\/span><\/td><\/tr>
                          Dolg \u010das segrevanja<\/span><\/span><\/td>Preizkus odpornosti grelnega elementa<\/span><\/span><\/td>Zamenjajte elemente, \u010de \uff1e15% variance<\/span><\/span><\/td><\/tr>
                          Okvara vakuuma<\/span><\/span><\/td>Izvedite preskus tesnosti (izguba \uff1c1 mmHg\/min)<\/span><\/span><\/td>Zamenjajte tesnilo vrat<\/span><\/span><\/td><\/tr><\/tbody><\/table><\/figure>
                          <\/div>

                          Oddelek Pogosta vpra\u0161anja<\/span><\/strong><\/span><\/h3>

                          V: Kako izra\u010dunati \u010das izpostavljenosti sterilizaciji?<\/span><\/strong><\/span> O: Uporabite formulo: <\/span><\/p>

                          \u00a0<\/div>
                          \ud835\udc61=(\ud835\udc37\ud835\udc63\ud835\udc4e\ud835\udc59\ud835\udc62\ud835\udc52\u00d7\ud835\udc3f\ud835\udc5c\ud835\udc54\ud835\udc410)+5 \ud835\udc5a\ud835\udc56\ud835\udc5b\ud835\udc60 \ud835\udc60\ud835\udc4e\ud835\udc53\ud835\udc52\ud835\udc61\ud835\udc66 \ud835\udc5a\ud835\udc4e\ud835\udc5f\ud835\udc54\ud835\udc56\ud835\udc5b<\/div>
                          \u00a0<\/div><\/div>

                          kjer D\u2081\u2082\u2081 = 1,5 min za kirur\u0161ke instrumente <\/span><\/p>

                          V: Ali lahko prekinem cikel avtoklava?<\/span><\/strong><\/span> O: Samo med fazo segrevanja. Ob prekinitvi sterilizacijske faze je potrebna popolna ponovna sterilizacija. <\/span><\/p>

                          V: Kako shranjevati sterilizirane pakete?<\/span><\/strong><\/span> O: Nadzorovano okolje: <\/span><\/p>

                          • Temperatura: 18-24\u00b0C <\/span><\/p><\/li>

                          • Vla\u017enost: \uff1c60% RH <\/span><\/p><\/li>

                          • Najdalj\u0161i rok trajanja: 180 dni <\/span><\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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                              Po\u0161ljite nam sporo\u010dilo<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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