Πρωτόκολλο λειτουργίας αυτόκλειστου: Διαδικασία αποστείρωσης βήμα προς βήμα

1. Προετοιμασία πριν από την αποστείρωση

1.1 Equipment Checklist

1.2 Load Preparation

• Packaging requirements:

  • Textile packs: ≤30 cm × 30 cm × 50 cm

  • Instrument trays: ≤7 kg dry weight

  • Liquid containers: ≤75% fill ratio

• Orientation rules:

   

  𝐴𝑖𝑟 𝑅𝑒𝑚𝑜𝑣𝑎𝑙 𝐸𝑓𝑓𝑖𝑐𝑖𝑒𝑛𝑐𝑦=1−𝜃90(𝜃=𝑡𝑖𝑙𝑡 𝑎𝑛𝑔𝑙𝑒)

   

  Maintain θ ≤15° for optimal air removal

2. Cycle Execution Protocol

2.1 Phase Parameters

2.2 Parameter Input Sequence

1. Select cycle type:

   • Gravity: 121°C/30min

   • Pre-vacuum: 132°C/4min

   • Liquid: 115°C/30min

2. Set safety margins:

    

   𝑇𝑠𝑒𝑡=𝑇𝑟𝑒𝑞+𝐶ℎ𝑎𝑚𝑏𝑒𝑟 𝑉𝑜𝑙𝑢𝑚𝑒(𝐿)/1000

    

   Example: 100L chamber at 121°C → 121 + 0.316 = 121.3°C

3. Initiate cycle with dual operator verification

3. Post-Cycle Validation

3.1 Immediate Checks

• Chamber drain filter inspection (＜1mL residual)

• Printout analysis:

  Παράμετρος	Acceptable Range
  F₀ value	≥15 minutes
  Temperature dip	＜0.3°C for ＜3 sec
  Pressure hold	±0.7 PSI from target

3.2 Biological Monitoring

• Geobacillus stearothermophilus test:

  • Incubation: 56-60°C for 24h

  • Population: 1×10⁶ spores/carrier

  • Pass criteria: No color change (pH indicator)

3.3 Load Release Criteria

1. Physical monitors passed

2. Chemical indicators color-matched

3. Biological results negative

4. Dryness confirmed (＜0.2% moisture)

Maintenance Protocol

Daily:

• Chamber cleaning with neutral pH detergent

• Door gasket inspection (replace if ＞0.2mm cracks)

Weekly:

• Steam trap functionality test

• Water conductivity measurement

Monthly:

• Thermocouple calibration (±0.25°C tolerance)

• Vacuum rate test (≥10 PSI/min)

Annual:

• Chamber pressure test (1.5×MAWP for 10min)

• Safety valve certification

Οδηγός αντιμετώπισης προβλημάτων

FAQ Section

Q: How to calculate sterilization exposure time? A: Use formula:

Where D₁₂₁ = 1.5 min for surgical instruments

Q: Can I interrupt an autoclave cycle? A: Only during heating phase. Aborting sterilization phase requires full re-sterilization.

Q: How to store sterilized packages? A: Controlled environment:

• Temperature: 18-24°C

• Humidity: ＜60% RH

• Maximum shelf life: 180 days