The core function uses high-pressure saturated steam to reach temperatures between [121°C] 그리고 [134°C] which destroys bacteria, viruses and spores.
주요 구성 요소:
Chamber (stainless steel, 316L grade)
Steam generator/boiler
Vacuum system (in advanced models)
Programmable logic controller (PLC)
In 1881 Charles Chamberland invented the initial medical autoclave for Louis Pasteur’s laboratory.
The year 1939 marked the introduction of gravity displacement autoclaves known as Class N.
During the 1980s pre-vacuum autoclaves (Class B) emerged as the solution for sterilizing hollow instruments.
2020s: IoT-enabled autoclaves with cloud-based cycle tracking.
Optimal heat transfer during sterilization requires steam to stay in its gaseous phase and avoid superheating.
Sterilization Formula: [ F_0 = \int_{0}^{t} 10^{(T-T_b)/z} \, dt ] (F_0) represents the effective sterilization time at 121°C ((T_b)) while (z) stands for the thermal resistance coefficient which usually measures about 10°C for bacterial spores.
| 매개변수 | Standard Range | Purpose |
|---|---|---|
| 온도 | 121°C – 134°C | Denatures microbial proteins |
| 압력 | 15 – 30 psi | Elevates steam temperature |
| 노출 시간 | 3 – 60 minutes | Ensures thermal death of pathogens |
| Vacuum Depth | -0.7 to -0.9 bar | Removes air for steam penetration |
헬스케어:
Surgical instrument sterilization (AAMI ST79 compliant)
Dental handpiece disinfection (validated 134°C/3min cycles)
실험실:
Decontaminating biohazard waste (WHO BSL-3 guidelines)
Preparing agar media (115°C/30min to prevent caramelization)
제약:
Vials and stoppers undergo sterilization through FDA 21 CFR 211.94 validated procedures.
Class N (Gravity Displacement) represents basic autoclave models which sterilize solid instruments.
The Class B (Pre-vacuum) autoclave is designed for sterilizing wrapped as well as hollow medical items according to EN 13060 standards.
Intermediate autoclave models that allow customized sterilization cycles belong to Class S.
Performance Comparison:
| 기능 | 클래스 N | Class B |
|---|---|---|
| Air Removal Method | Gravity | Vacuum Pump |
| 살균 시간 | 30–60 min | 15–30 min |
| Bowie-Dick Test | Not Required | Mandatory Daily Test |
Autoclaves stand as the top sterilization method because they effectively merge heat with pressure and steam penetration. Modern autoclaves maintain global standards adherence while enhancing operational effectiveness through advanced automation and monitoring systems.
Q1: How does an autoclave differ from a pressure cooker?
A: While both use steam under pressure, autoclaves have precision controls for temperature (±0.5°C), pressure sensors, and validation systems to meet medical sterilization standards.
Q2: Can paper be autoclaved? A: Paper can undergo autoclaving but must be placed in particular sterilization pouches that withstand 135°C conditions. Steam can become blocked when regular paper chars during the autoclaving process.
Q3: Why do some liquids explode during autoclaving? A: Containers filled above 75% capacity along with quick pressure release can lead to superheating. Always loosen lids and use borosilicate glass.
Q4: What’s the maintenance schedule for autoclaves? A: Daily: Door gasket inspection. Weekly: Chamber cleaning. Quarterly: Calibration of sensors. Annual: Pressure vessel certification (ASME BPVC required).
Q5: How to troubleshoot “Wet Loads” after sterilization? A: Increase the drying time and evaluate vacuum pump performance while verifying water separator operation. According to ANSI/AAMI ST79 guidelines wet packs must be classified as non-sterile.
오토클레이브 공정은 의료, 실험실 및 연구 시설에서 효과적인 멸균을 통해 유리 제품과 기구를 보호하기 위해 사용하는 필수 멸균 방식입니다. 고압 증기는 이 과정에서 병원균을 제거합니다.
